Affinity Media
Affinity chromatography media is popularly employed to purify biomolecules based on specific interactions to immobilised ligands showing affinity to each other. Highly pure products could be obtained in a single chromatography step.
We offer an extensive range of affinity chromatography media (resins) in lab packs, bulk volumes and prepacked columns. Depending on the nature of your application and the property of your feedstock, general selection guidance is shown along the products listed below.
Suitable use for Protein A & Protein G Media
Protein A SepFast; Protein A SepFast Large Beads; Protein A SepFast HighRes; Protein A SuperSpin – Standard affinity media for purifying antibodies.
Protein A SepFast Endure; Protein A SepFast Large Beads Endure; Protein A SepFast HighRes Endure – Affinity media for purifying antibodies. Alkaline-stable (0.5M NaOH), good for cleaning & sanitisation at process scale.
Protein G SepFast; Protein G SepFast Large Beads; Protein G SepFast HighRes – Having affinity to wider sources / classes of antibodies than Protein A.
Protein A SepFast MAG; Protein G SepFast MAG – Magnetic media. Suitable for micro-scale and high-throughput use.
Suitable use for Immobilised Metal Affinity Media
IDA SepFast; IDA SepFast HighRes – Low cost chelating media.
IMAC SepFast; Ni SepFast; Co SepFast; Zn SepFast; Ready-to-use His Buffer Kit – For purifying histidine-tagged proteins. High binding capacity, low metal leakage.
IMAC SepFast Large Beads; Ni SepFast Large Beads; Co SepFast Large Beads; Zn SepFast Large Beads; Ready-to-use His Buffer Kit – For purifying histidine-tagged proteins from viscous or unclarified sources. High binding capacity, low metal leakage.
IMAC SepFast HighRes; Ni SepFast HighRes; Co SepFast HighRes; Zn SepFast HighRes; Ni SuperSpin; Co SuperSpin, Zn SuperSpin; Ready-to-use His Buffer Kit – For high resolution or faster purification of histidine-tagged proteins. High binding capacity, low metal leakage.
Ni SepFast MAG; Co SepFast MAG; Zn SepFast MAG; Ready-to-use His Buffer Kit – Magnetic media. Suitable for micro-scale and high-throughput use.
Suitable use for Heparin Media
Heparin SepFast; Heparin SepFast HighRes – For purifying plasma proteins and DNA-binding proteins such as polymerases.
Suitable use for Maltose-binding-protein Media
MBP SepFast; MBP SepFast HighRes – For purifying proteins with maltose-binding-protein tag.
Suitable use for Glutathione Media
Glutathione SepFast; Glutathione SepFast MAG – For purifying proteins with glutathione-S-transferase (GST) tag; the magnetic version is good for high throughput applications.
Suitable use for Con A Media
Con A SepFast 4B – For purifying glycoproteins, polysaccharides, and glycolipids. Con A binds to molecules containing α-d-mannopyranosyl, α-d-glucopyranosyl, and sterically related residues.
Suitable use for Streptavidin Media
Streptavidin SepFast; Streptavidin SepFast MAG – For capturing biotinylated molecules; the magnetic version is good for high throughput applications.
Suitable use for PlasmidPure Media
PlasmidPure HighRes – For purifying supercoiled plasmids.
Affinity Media products
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Con A SepFast 4B
£93.17 – £745.36 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
Glutathione Media
£69.85 – £6,288.98 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
Heparin SepFast Media
£191.47 – £4,382.62 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
IDA SepFast
£166.98 – £2,180.42 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
IMAC SepFast
£125.76 – £4,472.16 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
IMAC SepFast HighRes
£164.08 – £5,869.71 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
IMAC SepFast Large Beads
£164.08 – £4,472.16 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
Magnetic IMAC Resins
£130.31 – £164.08 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
MBP (Maltose Binding Protein) Media
£54.57 – £4,840.00 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
PlasmidPure HighRes
£107.81 – £2,393.14 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
Protein A SepFast Endure Media
£149.69 – £10,900.89 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
Protein A SepFast MAG and Protein G SepFast MAG
£97.88 – £628.90 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
Protein A SepFast Media
£120.34 – £9,528.75 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
Protein G SepFast Media
£156.79 – £10,482.23 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page -
Streptavidin Media
£198.00 – £869.00 ex. VAT Select options This product has multiple variants. The options may be chosen on the product page