Description
IMAC SepFast Large Beads is designed for the purification of histidine-tagged proteins in batch, gravity flow or packed bed operations from unclarified or very viscous samples.
Key benefits include:
- Good for processing viscous or unclarified feeding materials
- Low cost due to the novel manufacturing processes
- High binding capacity (could be over 40 mg/ml)
- Fast and easy protein purification from unclarified or clarified samples
- Choice of three pre-charged metal ions
- Very low metal leakage
- Full protein purification manual supplied
Clarified or unclarified cell lysates (for intracellular proteins) or culture broths (for extracellular proteins) can be directly processed with IMAC SepFast Large Beads in packed bed format without concern of bed blockage. Also, its high mechanical strength permits liquid to pass through a column at excellent flow rates. All these translate into high process flexibility and higher protein yield at a shortened purification time.
IMAC SepFast Large Beads or its three metal charged formats i.e. Ni SepFast Large Beads, Co SepFast Large Beads and Zn SepFast Large Beads, are highly stable and compatible with a wide range of chemicals (e.g. detergents, denaturing reagents and reducing reagents etc) commonly used in protein purification processes, which means that more cost-efficient operations can be developed for the best performance. The product is designed for process scale use. Regulatory support files are available on request.