Description
MBP SepFast and MBP SepFast HighRes are chromatography media for purifying recombinant proteins tagged with maltose binding protein (MBP).
Tagging proteins with MBP often gives increased expression levels and higher solubility of target proteins. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. With the benefit of increased solubility, the tag could be particularly useful for recombinant proteins expressed in an insoluble form (inclusion bodies).
Affinity purification using MBP SepFast media takes place under physiological conditions and mild elution is performed using maltose. This preserves the activity of the target protein. Even intact protein complexes may be purified. In addition, the high specificity of the binding means that very high purity can be achieved in just one step in combination with high binding capacity.
MBP SepFast media is a robust, high resolution product based on well known agarose beads. In particular, the small and evenly sized beads of MBP SepFast HighRes ensure that MBP-tagged proteins elute in narrow peaks, thus minimizing the need for further concentration steps. MBP SepFast tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same medium to be used for repeated purifications.
The base matrix is made of highly cross-linked porous agarose. The particle has open pore structure with good mass transfer property to large protein molecules. The media shows high mechanical rigidity. So it can be operated at high flow velocities with moderate pressure drop.
These products are suitable for process scale use. Regulatory support files are available on request.