Description
IDA SepFast and IDA SepFast HighRes are specifically designed and fabricated for low-cost purification of histidine-tagged proteins. The base matrix is made of heavily cross-linked agarose. Its high mechanical strength permits liquid passing through columns at decent flow rates. The ligand is of iminoacetic acid which is the first generation chelating ligand particularly good with copper ion charged.
IDA SepFast is suitable for preparative scale to industrial scale packed column operations. The resin can be readily scaled up for mass purification of proteins at reduced cost.
IDA SpFast HighRes is good for small to pilot scale purification use where higher dynamic binding capacity at fast flow rates is desired.
Pre-packed columns for screening and process development use are available at following sizes: 1 ml, 5 ml, 3.8 ml (7x100mm ID x BH), 9.5 ml (11x100mm ID x BH), 20 ml (16x100mm ID x BH), 53 ml (26x100mm ID x BH). All these columns have connections fully compatible to Akta systems. Column hardware has 6 bar (0.6 MPa) pressure rating. See Column Instruction for details of HiSep and Optio column hardware.
Pre-packed columns for pilot to production scale use with column diameter ranging from 5cm to 45cm are available on request. For bulk loose media, please contact us.
We support large scale production use. Regulatory support files are available on request.
For higher protein binding capacity, purity or less metal leakage, alternative products are IMAC SepFast series (i.e. NTA-type chelating ligand).