Description
IMAC SepFast is designed for the purification of histidine-tagged proteins in batch, gravity flow, packed bed, or 96-well microplate operations.
Key benefits:
- Low cost due to the novel manufacturing processes
- High binding capacity (could be over 40 mg/ml)
- Fast and easy protein purification from unclarified or clarified samples
- Choice of three pre-charged metal ions
- Very low metal leakage
- Full protein purification manual supplied
Its carefully controlled pore structure allows fast access of the target protein to the immobilised metal ligand. Also, its high mechanical strength permits liquid to pass through a column at excellent flow rates. Clarified or unclarified cell lysates (for intracellular proteins) or culture broths (for extracellular proteins) can be directly processed with IMAC SepFast. All these translate into high process flexibility and higher protein yield at a shortened purification time.
IMAC SepFast or its three metal charged formats i.e. Ni SepFast, Co SepFast and Zn SepFast, are highly stable and compatible with a wide range of chemicals (e.g. detergents, denaturing reagents and reducing reagents etc) commonly used in protein purification processes, which means that more flexible operations can be developed for the best performance.
Pre-packed columns for screening and process development use are available at following sizes: 1 ml, 5 ml, 3.8 ml (7x100mm ID x BH), 9.5 ml (11x100mm ID x BH), 20 ml (16x100mm ID x BH), 53 ml (26x100mm ID x BH). All these columns have connections fully compatible to Akta systems. Column hardware has 6 bar (0.6 MPa) pressure rating. See Column Instruction for details of HiSep and Optio column hardware.
Pre-packed columns for pilot to production scale use with column diameter ranging from 5cm to 45cm are available on request. For bulk loose media, please contact us.
More information about IMAC SepFast.