Description
SepFast DUO 150 range is unique ion-exchange chromatography media utilising dual functionalities. Individual beads are coated with an inert polymer out-layer giving a size-exclusion effect to monoclonal antibodies. Inside the bead are anion-exchange or cation-exchange ligands. This type of novel media is designed for the selective purification of monoclonal antibodies or other molecules of similar size.
The media can be packed in a column where monoclonal antibodies will pass through the column bed and collected in flow through fraction, whilst low molecular weight impurities will be captured within the beads. This affords a very gentle purification process resulting in efficient clean up and high recovery of active antibodies.
The base matrix is a composite of polysaccharides that have been highly cross-linked. The media is stable in most of the chemical conditions experienced in bioprocessing industry. SepFast DUO 150 can be used at process scale. Regulatory support files are available.
Pre-packed columns for screening and process development use are available at following sizes: 1 ml, 5 ml, 3.8 ml (7x100mm ID x BH), 9.5 ml (11x100mm ID x BH), 20 ml (16x100mm ID x BH), 53 ml (26x100mm ID x BH). All these columns have connections fully compatible to Akta systems. Column hardware has 6 bar (0.6 MPa) pressure rating. See Column Instruction for details of HiSep and Optio column hardware.
Pre-packed columns for pilot to production scale use with column diameter ranging from 5cm to 45cm are available on request. For bulk loose media, please contact us.
The range of SepFast DUO ion-exchange media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.