Ion Exchange (IEX) media

IEX SepFast Large Beads

£66.03£1,038.40 ex. VAT

Description

Q , DEAE , SP and CM SepFast Large Beads are a group of ion exchange chromatography media with large particle sizes (100 – 350 μm). The base matrix shows excellent flow property and stable physical property.

The above ion-exchange chromatography media is particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.

This group of ion-exchange media has a balanced design among ligand density, loading capacity and separation power of individual components for large-scale biomanufacturing applications.

The base matrix of SepFast Large Beads is made of highly cross-linked agarose with excellent flow property. They are very stable to most of the chemical conditions experienced in the bioprocessing industry.

Further information about IEX SepFast Large Beads.

Characteristics of IEX SepFast Large Beads

Matrix

Highly cross-linked agarose beads

Functional Group

Quaternary ammonium strong anion (Q SepFast Large Beads)

Diethylaminoethyl weak anion (DEAE SepFast Large Beads)

Sulfopropyl strong cation (SP SepFast Large Beads)

Carboxymethyl weak cation (CM SepFast Large Beads)

Total Ionic Capacity

0.12-0.2 mmol/ml (Q SepFast Large Beads)

0.08-0.13 mmol/ml (DEAE SepFast Large Beads)

0.15-0.25 mmol/ml (SP SepFast Large Beads)

0.08-0.13 mmol/ml (CM SepFast Large Beads)

Particle Size

100 – 350 μm

Operational Flow Velocity*

>2000 cm/hr

*Measured in a 32 mm ID column at a bed height of 15 cm using water at room temperature.

pH Stability

2-14 (short term) and 3-12 (long term) (Q SepFast Large Beads and DEAE SepFast Large Beads)

2-14 (short term) and 4-12 (long term) (SP SepFast Large Beads and CM SepFast Large Beads)

Working Temperature

+4°C – +30°C

Chemical Stability

All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol

Avoid

Oxidizing agents, anionic detergents (Q SepFast Large Beads and DEAE SepFast Large Beads)

Oxidizing agents, cationic detergents (SP SepFast Large Beads and CM SepFast Large Beads)

Storage

20% ethanol (Q SepFast Large Beads)

20% ethanol (DEAE SepFast Large Beads)

20% ethanol + 0.2M sodium acetate (SP SepFast Large Beads)

20% ethanol (CM SepFast Large Beads)