DNA and mRNA Purification
Based on our unique shelled bead technology, SepFast DUO range of ion-exchange resins can be used to remove DNA / RNA fragments, or to remove impurities from mRNA after IVT reaction.
SepFast DUO IEX is unique ion-exchange chromatography media utilising dual functionalities. Individual beads are coated with an inert polymer out-layer giving a size- exclusion effect. Inside the bead are anion-exchange or cation-exchange ligands. This type of novel media is designed for the selective purification of target DNA or mRNA molecules based on both molecular weights and charges. Bound materials (impurities) such as
enzymes or DNA fragments / nucleotides can be recovered (if the purification processdesires) via elution step or discarded via cleaning and regeneration step.
The media is stable in most of the chemical conditions experienced in bioprocessing industry. Regulatory support file is available to support large scale manufacturing use.
SepFast™ DUO 5000 Q and SepFast™ DUO 6000 Q – suitable for removing reaction enzymes, un-reacted reagents or fragments in synthetic DNA and mRNA.
SepFast™ DUO 7000 Q – could be used to remove DNA fragments up to 500 – 1000 bp.
SepFast™ DUO 8000 Q – could be used to remove DNA fragments up to 1 – 1.5 kb.
SepFast™ DUO 9000 Q – could be used to remove DNA fragments up to 1.5 – 2 kb.
SepFast™ DUO 10000 Q – could be used to remove DNA fragments > 2 kb.
ViralPolish chromatography media is a class of dual layer polymer beads possessing an inert external shell with tightly controlled pore size and internal polyfunctional ligands for stronger binding of impurities (particularly to enzymes). ViralPolish media is more salt tolerant than SepFast DUO ion-exchange media.
Large molecules, such as high molecular DNA and mRNA, are excluded from the beads. The beads can be packed in a column where large DNA or mRNA will pass through the. column bed and collected in flow through fraction, whilst small impurities such as enzymes and DNA fragments will be captured within the beads. This affords a very gentle purification process resulting in efficient clean up and high recovery of active products.
ViralPolish is available in a range of outer shells (i.e. different pore sizes).
The media is stable in most of the chemical conditions experienced in bioprocessing industry. Regulatory support file is available to support large scale manufacturing use.
ViralPolish™ 5000B and ViralPolish™ 6000B – suitable for removing reaction enzymes, un-reacted reagents or fragments in synthetic DNA and mRNA.
ViralPolish™ 7000B – could be used to remove DNA fragments up to 500 – 1000 bp.
ViralPolish™ 8000B – could be used to remove DNA fragments up to 1 – 1.5 kb.
ViralPolish™ 9000B – could be used to remove DNA fragments up to 1.5 – 2 kb.
ViralPolish™ 10000B – could be used to remove DNA fragments > 2 kb.