Description
Based on the principle behind size exclusion chromatography, cross-linked neutral dextran particles with very tight pores are packed into columns for rapid desalting and / or buffer exchange.
SuperSpin Desaltor is designed for desalting small samples at speed. Up to 24 samples (depending on the type of microcentrifuge) of 10 – 100 µl can be processed in one spin. It is a much faster and more efficient approach when compared to dialysis tubes or membrane ultrafiltration. The small porous particles provide huge surface area with very short diffusion distance, which means small molecules such as salts can be partitioned rapidly. In comparison, both dialysis tubes and membranes have very low surface areas. Dialysis is also incredibly time consuming, and membranes often suffer blockages resulting in a loss of valuable material.
Key benefits:
- Rapid desalting or buffer exchange
- Very little loss of target molecules (typically > 95% recovery)
- Most proteins (> 6,000 dalton) and DNAs (> 10 bp) can be desalted
- DNAse free
- Full operation manual supplied
SuperSpin Desaltor is particularly useful for the following applications:
- Desalting of histidine-tagged proteins (e.g. imidazole and NaCl) recovered from IMAC SuperSpin
- Desalting of samples before loading to SDS-PAGE
- Desalting of samples before conducting other analysis
- Buffer exchange, for example, after low pH elution
- Desalting of DNAs
SuperSpin Concentrator is designed for desalting and concentrating 200 μl to 220 small samples at speed. The concentration factor is around 5.
Pre-packed columns for desalting / buffer exchange use are available at following sizes: 1 ml, 5 ml, 9.5 ml (11x100mm ID x BH), 20 ml (16x100mm ID x BH), 53 ml (26x100mm ID x BH). Up to 30% column volume of sample can be loaded to a given column. All these columns have connections fully compatible to Akta systems. Column hardware has 6 bar (0.6 MPa) pressure rating. Pre-packed columns for pilot to production scale use with column diameter ranging from 5cm to 45cm are available on request.
More information about SuperSpin Desaltor.
More information about SuperSpin Concentrator.