Gel Filtration (SEC) Media

Cross-Linked Agarose Beads

£174.70£866.48 ex. VAT

Description

SepFast CL-2B, CL-4B and CL-6B range of chromatography media is often used for fractionation of large biological molecules based on their molecular weights. Gel Filtration is a proven technique, which is widely used for size-based molecular separation.  It is also used as good base matrix to immobilise affinity ligands.

The materiel is very stable to most of the chemical conditions experienced in the bioprocessing industry.

More information about cross-linked agarose beads.

Characteristics of Cross-Linked Agarose Beads

Matrix

CL-2B: cross-linked 2% agarose bead

CL-4B: cross-linked 4% agarose bead

CL-6B: cross-linked 6% agarose bead

Particle Size

50 – 150 µm

Operational Pressure

Max. 0.5 bar (0.05 MPa, 7 psi)

Operating Flow Velocity

Normally 10-50 cm/hour

pH Stability

2-13 (short term) and 3-12 (long term)

Working Temperature

4°C – 30°C

Chemical Stability

All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol

Avoid

Oxidizing agents

Storage

20% ethanol or 10 mM NaOH